Method Abstract #87, Vitamin C

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Vitamin C

Method Abstract #87

Scope and Application

This method determines the ascorbic acid content of aqueous solutions. This method conforms to AOAC Official Method 967.21.

Method Summary

This method involves the preparation of a 2:5 solution of ascorbic acid and metaphosphoric acid-acetic acid. This is done by combining the ascorbic acid sample with metaphosphoric acid-acetic acid. The 2:5 sample solution is then titrated to an inflection endpoint with Indophenol. The ascorbic acid content can then be calculated.

Sample Titration Curve

Method Performance

*Data for this measuring range was obtained using laboratory prepared standards formulated from potassium chloride. The measuring range may be increased by using auto-dilution.

**The Method Detection Limit (MDL) was determined based on data obtaining a coefficient of variance better than 30%. Results may differ depending on laboratory practices and sample matrix.

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