Biochemical oxygen demand bottles in a 54 place MANTECH autosampler application abstract

Automated Biochemical Oxygen Demand (BOD) Analysis Solutions

MANTECH has a variety of biochemical oxygen demand (BOD) systems to best suit the needs of your laboratory. Whether you need manual or automated, big or small, simple or complex, MANTECH can create the ideal BOD solution for your laboratory. MANTECH’s Automated BOD analysis systems are robust, come with easy to use software, and provide accurate results that stand the test of time.

Download the BOD method abstract.

NEW in 2020:  Automated BOD with 10 minute BOD estimator Packages – ALL in ONE

BOD Analyzer Benefits

  • Automates the 5-day and 7-day BOD and CBOD standard analysis methods. Conforms to ASTM Standard Methods 5210B, ISO EN 18991 & ISO EN 18992

  • Eliminates potential for human error by automating up to five pumps for reagent addition

  • Customizable user interface to simplify operation

  • Easily manage and prioritize samples during analysis

  • Quickly identify results with barcoded labels

AM402_BOD
The MANTECH Automated BOD system is an accurate and efficient method to process BOD and CBOD. I have found it especially handy with large sample loads. The most impressive part of MANTECH is the irrefutable customer service. If there is ever a question, problem, or any form of maintenance, these are the people I would want helping me.
What I like most about the instrument is that it allows me to have the freedom to do other things.
The system gives us a safer environment and equipment, increased capacity, the ability to automatically deliver the dilution water, updated software and better usage of analyst time.

BOD Analyzer Models and Specifications

MANTECH’s next generation autosamplers are quieter, faster and more accurate.

System Features:

  • 18, 36, 54, 72 and 90 positions x300ml BOD bottle capacity models
  • Automated dilution, seed & inhibitor
  • Can be coupled with MANTECH’s Automated Titration Analysis for a two-in-one system configuration

Biochemical oxygen demand system with 3 pumps.

With a low purchase price and small footprint, this system is ideal for smaller laboratories who analyse a few samples per month.

System Features:

  • 18 place bottle capacity
  • Manual operation

All of our systems can be customized to suit your needs. Contact us for more information.

Video Gallery

Frequently Asked Questions

BOD FAQ

  • What is the weight and dimensions for AM400 autosamplers?
    Autosampler Model Dimensions in Inches Dimensions in Centimeters Crate ONLY (in lbs) Crate ONLY (in kg) Weight Estimates with System & Accessories (in lbs)* Weight Estimates with System & Accessories (in kg)*
    401 30 x 35 x 25 76 x 89 x 64 75 34 200 91
    402 42 x 35 x 25 107 x 89 x 64 91 41 250 113
    403 53 x 35 x 25 135 x 89 x 64 107 49 275 125
    404 66 x 35 x 25 168 x 89 x 64 118 54 300 136
    405 79 x 35 x 25 201 x 89 x 64 130 59 325 148

    *Actual weight may vary by actual system and accessories.

  • How do you calculate seed correction and BOD?

    To learn how to calculate Seed Correction and BOD, please refer to this document. In BOD Pro, two separate values for Seed Correction Factor are stored along with the Run; one is referred to as BODSeedCorrection, and the other CBODSeedCorrection. The way we calculated BODSeedCorrection in BOD Pro is as follows:

    1. Dividing the depletion of each BOD Seed bottle by its own volume (the seed volume), the resulting quantity is referred to as DepletionPerMLSeed
    2. and then we average the DepletionPerMLSeed over all the BOD Seed bottles.

    CBOD Seed Correction was calculated by doing the same on CBOD Seed bottles. The software allows for only one Seed ID to be included in the Run. There can be any number of BOD or CBOD Seed bottles within that Seed ID group.

  • What does high BOD indicate?

    BOD indicates the amount of putrescible organic matter present in water. Therefore, a low BOD is an indicator of good quality water, while a high BOD indicates polluted water. Dissolved oxygen (DO) is consumed by bacteria when large amounts of organic matter from sewage or other discharges are present in the water.

  • What is the BOD of raw sewage?

    BOD can also be defined as the amount of oxygen required by the micro-organisms in the stabilization of organic matter. The results are generally expressed as the amount of oxygen taken by one litre sample (diluted with aerated water) when incubated at 20 degree for five days. BOD of raw sewage is 300-600 mg/litre.

  • What is biochemical oxygen demand?

    Biochemical Oxygen Demand (BOD), also often referred to as biological oxygen demand, is a test performed to measure the potential of wastewater and other waters to deplete the oxygen level of receiving waters. In other words, the BOD test is performed to determine what effect dirty water, containing bacteria and organic materials, will have on animal and plant life when released into a stream or lake. Learn more here.

  • What is the measuring range for Dissolved Oxygen on MANTECH systems?

    MANTECH systems utilize one of two dissolved oxygen probes from YSI for automated dissolved oxygen determination. The measuring ranges of both probes are listed below, along with a link to the complete specification sheets.

    YSI FDO 4410 IDS Sensor

    • Dissolved Oxygen Range: 0 to 20 mg/L
    • Air Saturation Range: 0 to 200%
    • Temperature Measurement Range: 0 to 50°C
    • Technical Specifications

    YSI ProOBOD Sensor

    • Dissolved Oxygen Range: 0 to 50 mg/L
    • Air Saturation Range: 0 to 500%
    • Temperature Measurement Range: Ambient 10 to 40°C; Compensation -5 to 50°C
    • Technical Specifications

     

  • How does the PeCOD method compare to BOD, TOC, and conventional COD?

    There are several common methods to test wastewater and drinking water for organic pollutants, natural and chemical.  Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD) and, Total Organic Carbon (TOC) compromise the three main methods of testing water samples.  BOD and COD methods differ from TOC because they measure the amount of oxygen that is depleted by organic species in water.  Moreover, TOC is a measure of all carbon (both organic and inorganic), rather than the oxygen that is reduced by these species.  As written by a TOC manufacturer, “TOC on its own sheds no light on the oxidizability of the measured carbon or the amount of oxygen needed for its biodegradation.  Specific to COD, it measures the reactive fraction of the TOC.  This is also known as oxidizability in the European Union.

    View the full article: Comparison of COD, BOD and TOC Methods for organics, which summarizes the advantages and disadvantages of the COD, BOD and TOC methods, and compares them to the PeCOD method.

     

  • How do I retrieve calibration records from my YSI 4010 MultiLab IDS meter?

    The YSI 4010 MultiLab IDS meter will store the records for the last 10 calibrations performed on the meter. The instructions for retrieving these calibrations can be found on Technical Bulletin 2021 – 010.

  • What does high BOD indicate?

    BOD indicates the amount of putrescible organic matter present in water. Therefore, a low BOD is an indicator of good quality water, while a high BOD indicates polluted water. Dissolved oxygen (DO) is consumed by bacteria when large amounts of organic matter from sewage or other discharges are present in the water.

  • What is the BOD of raw sewage?

    BOD can also be defined as the amount of oxygen required by the micro-organisms in the stabilization of organic matter. The results are generally expressed as the amount of oxygen taken by one litre sample (diluted with aerated water) when incubated at 20 degree for five days. BOD of raw sewage is 300-600 mg/litre.

  • What is the COD to BOD ratio in domestic sewage influent?

    BMS have recorded average ratios of 2-3 mg/l COD to 1 mg/l BOD over its 30 years of business. Influent COD in normal domestic sewage is therefore generally 600 – 900 mg/l and it is then treated to at least 30 -100 mg/l before discharge to minimize pollution potential.

  • How does PC-BOD calculate BOD when samples have failed BOD Method criteria?
    • If all samples fail the final DO rule, the software will report the value for the sample with the highest dilution (lowest sample volume) and display a greater than (>) symbol.
    • If all samples in a set fail the depletion rule and the BOD of the bottle with the lowest dilution (highest sample volume) is greater than the minimum detection, the software will report the value for the sample with the lowest dilution (highest sample volume) and display a less than (<) symbol.
    • A less than symbol (<) will appear when all samples in a set have a depletion of less than that specified in the BOD Method.  If the BOD of the bottle with the lowest dilution (highest sample volume) is less than the minimum detection for a given sample condition, the average will be displayed as follows:
      • Diluted samples with no seed added < 2 mg/L
      • Diluted samples with seed added < 1 mg/L
      • Undiluted samples with no seed added < 0.1 mg/L
      • Undiluted sampled with seed added < 0.0 mg/L

     

  • How long does it take to measure initials and finals on a MANTECH AM300 series multi-rack system?

    The length of time to measure initials and finals on a MANTECH AM300 series multi-rack system varies depending on the method of sample preparation, sampler size, and probe style. For an example of a typical MANTECH BOD system click here.  For an individualized estimate contact us at support@mantech-inc.com.

  • What information is required to automate biochemical oxygen demand?

    Automating the biochemical oxygen demand (BOD) test can greatly increase the efficiency and production capacity of a laboratory. In order to automate this analysis, the following information is required:

    • How many bottles on average do you set up for an initials batch?
      • (For example, this accounts for all samples and all dilutions per sample, plus blanks, GGA’s, etc.)
    • On what days of the week do you set up for initials?
      • (For example, many laboratories set up Initials on Wednesday, Thursday, and Friday then Finals on Monday, Tuesday, and Wednesday since the laboratory is closed on weekends)
    • What is the maximum number of bottles in your incubator at any one time?
    • Do you analyze BOD, cBOD, or both?
    • What is the range of BOD concentration?
    • What type of samples are you analyzing?
      • (For example: industry type, river, etc.)
    • Who are the clients or users of the BOD information?
      • For example industrial pre-treatment, WWTP effluent compliance, WWTP engineers, etc.

     

  • What are the dimensions of MANTECH BOD Autosampler Racks?
    Part Number Rack Size Bottle Style Width Depth Rack Height Rack Height w/ Bottles
    PB-10138 11 x 300mL Plastic

    337 mm

    13.25 inches

    234 mm

    9.19 inches

    88 mm

    3.13 inches

    8 mm + bottle height

    0.313 inches + bottle height

    PB-10139 11 x 300mL Glass

    337 mm

    13.25 inches

    234 mm

    9.19 inches

    88 mm

    3.13 inches

    8 mm + bottle height

    0.313 inches + bottle height

    PB-10162 23 x 300mL Glass

    488 mm

    19 inches

    307 mm

    12.06 inches

    71 mm

    3.81 inches

    9.5 mm + bottle height

    0.375 inches + bottle height

    PB-10163 23 x 300mL Plastic

    488 mm

    19 inches

    307 mm

    12.06 inches

    71 mm

    3.81 inches

    9.5 mm + bottle height

    0.375 inches + bottle height

    PB-10060 (discontinued) 24 x 300mL Glass/Plastic

    485 mm

    18.82 inches

    315 mm

    12.38 inches

    193 mm

    7.60 inches

    5 mm + bottle height

    0.188 inches + bottle height

    PB-10133 (discontinued) 24 x 300mL Glass/Plastic

    485 mm

    18.82 inches

    315 mm

    12.38 inches

    131 mm

    5.125 inches

    5 mm + bottle height

    0.188 inches + bottle height

    PB-10185 18 x 300mL Glass

    487.68 mm

    19.2 inches

    236.22 mm

    9.03 inches

    63.5 mm

    2.5 inches

    2.69 mm + bottle height

    0.106 inches + bottle height

    PB-10186 18 x 300mL Plastic 487.68 mm

    19.2 inches

    236.22 mm

    9.03 inches

    63.5 mm

    2.5 inches

    2.69 mm + bottle height

    0.106 inches + bottle height

     

  • How do I configure the YSI 4010 MultiLab IDS meter for PC-BOD?

    To use the YSI 4010 MultiLab IDS meter with PC-BOD, the appropriate driver, computer settings, and software settings must be applied.  Download the Technical Bulletin for more details.

  • Why does the barometer reading on my YSI 4010 MultiLab IDS meter not match the reading documented by my local weather service?

    The YSI MultiLab IDS meter outputs a true barometric pressure reading of its location, which is dependent on elevation above sea level.

    Local weather services typically use a corrected barometric pressure reading, which corrects the reading to sea level.

    To approximate the true barometric pressure reading from a corrected barometric pressure reading, use the following equation:

    True barometric pressure [mmHg] = Corrected barometric pressure [mmHg] – (0.025 * local altitude [feet] )

  • Can I calibrate the internal barometer on my meter?

    The internal barometer on the YSI MultiLab IDS meter is calibrated upon manufacturing and is designed to last the lifespan of the barometer sensor. The sensor is very stable and can last many years. Standard Methods does not specify a requirement for an adjustable barometer for dissolved oxygen measurements or biological oxygen demand testing.

     

  • How do I calibrate my DO probe with the YSI 4010 MultiLab IDS Meter?

    Unless otherwise specified in company or site-specific procedures, specifications, and regulations, a water vapor-air saturated calibration should be sufficient for probe calibration.  For full calibration procedures, please refer to the following pdf.

  • How do I set the sensor cap coefficients in my YSI 4010 MultiLab IDS meter?

    Follow the step-by-step instructions in the pdf.

  • How do I set the date and time in my YSI 4010 MultiLab IDS Meter?

    Follow the step-by-step instructions in the pdf.

  • What is the difference between BOD and cBOD?

    Biochemical oxygen demand (BOD) is a way to assess the amount of oxygen required for aerobic microorganisms to decompose the organic material in a sample of water over a specific time frame. It is the oxygen uptake demand of a source of water. Carbonaceous biochemical oxygen demand (CBOD) is the same method as BOD, but the nitrifying bacteria in the sample are inhibited. Nitrifying bacteria consume nitrogenous materials (compounds with reduced forms of nitrogen) and add to the oxygen demand of the wastewater. Nitrogenous materials are often seen as interference because the purpose of the BOD test is to measure carbonaceous material.

  • Biochemical Oxygen Demand (BOD), also often referred to as biological oxygen demand, is a test performed to measure the potential of wastewater and other waters to deplete the oxygen level of receiving waters. In other words, the BOD test is performed to determine what effect dirty water, containing bacteria and organic materials, will have on animal and plant life when released into a stream or lake. Learn more here.

    Automating the biochemical oxygen demand (BOD) test can greatly increase the efficiency and production capacity of a laboratory. In order to automate this analysis, the following information is required:

    • How many bottles on average do you set up for an initials batch?
      • (For example, this accounts for all samples and all dilutions per sample, plus blanks, GGA’s, etc.)
    • On what days of the week do you set up for initials?
      • (For example, many laboratories set up Initials on Wednesday, Thursday, and Friday then Finals on Monday, Tuesday, and Wednesday since the laboratory is closed on weekends)
    • What is the maximum number of bottles in your incubator at any one time?
    • Do you analyze BOD, cBOD, or both?
    • What is the range of BOD concentration?
    • What type of samples are you analyzing?
      • (For example: industry type, river, etc.)
    • Who are the clients or users of the BOD information?
      • For example industrial pre-treatment, WWTP effluent compliance, WWTP engineers, etc.
    Part Number Rack Size Bottle Style Width Depth Rack Height Rack Height w/ Bottles
    PB-10138 11 x 300mL Plastic

    337 mm

    13.25 inches

    234 mm

    9.19 inches

    88 mm

    3.13 inches

    8 mm + bottle height

    0.313 inches + bottle height

    PB-10139 11 x 300mL Glass

    337 mm

    13.25 inches

    234 mm

    9.19 inches

    88 mm

    3.13 inches

    8 mm + bottle height

    0.313 inches + bottle height

    PB-10162 23 x 300mL Glass

    488 mm

    19 inches

    307 mm

    12.06 inches

    71 mm

    3.81 inches

    9.5 mm + bottle height

    0.375 inches + bottle height

    PB-10163 23 x 300mL Plastic

    488 mm

    19 inches

    307 mm

    12.06 inches

    71 mm

    3.81 inches

    9.5 mm + bottle height

    0.375 inches + bottle height

    PB-10060 (discontinued) 24 x 300mL Glass/Plastic

    485 mm

    18.82 inches

    315 mm

    12.38 inches

    193 mm

    7.60 inches

    5 mm + bottle height

    0.188 inches + bottle height

    PB-10133 (discontinued) 24 x 300mL Glass/Plastic

    485 mm

    18.82 inches

    315 mm

    12.38 inches

    131 mm

    5.125 inches

    5 mm + bottle height

    0.188 inches + bottle height

    PB-10185 18 x 300mL Glass

    487.68 mm

    19.2 inches

    236.22 mm

    9.03 inches

    63.5 mm

    2.5 inches

    2.69 mm + bottle height

    0.106 inches + bottle height

    PB-10186 18 x 300mL Plastic 487.68 mm

    19.2 inches

    236.22 mm

    9.03 inches

    63.5 mm

    2.5 inches

    2.69 mm + bottle height

    0.106 inches + bottle height

    The length of time to measure initials and finals on a MANTECH AM300 series multi-rack system varies depending on the method of sample preparation, sampler size, and probe style. For an example of a typical MANTECH BOD system click here.  For an individualized estimate contact us at support@mantech-inc.com.

    Biochemical oxygen demand (BOD) is a way to assess the amount of oxygen required for aerobic microorganisms to decompose the organic material in a sample of water over a specific time frame. It is the oxygen uptake demand of a source of water. Carbonaceous biochemical oxygen demand (CBOD) is the same method as BOD, but the nitrifying bacteria in the sample are inhibited. Nitrifying bacteria consume nitrogenous materials (compounds with reduced forms of nitrogen) and add to the oxygen demand of the wastewater. Nitrogenous materials are often seen as interference because the purpose of the BOD test is to measure carbonaceous material.

    Follow the step-by-step instructions in the pdf.

    Unless otherwise specified in company or site-specific procedures, specifications, and regulations, a water vapor-air saturated calibration should be sufficient for probe calibration.  For full calibration procedures, please refer to the following pdf.

    The internal barometer on the YSI MultiLab IDS meter is calibrated upon manufacturing and is designed to last the lifespan of the barometer sensor. The sensor is very stable and can last many years. Standard Methods does not specify a requirement for an adjustable barometer for dissolved oxygen measurements or biological oxygen demand testing.

    The YSI MultiLab IDS meter outputs a true barometric pressure reading of its location, which is dependent on elevation above sea level.

    Local weather services typically use a corrected barometric pressure reading, which corrects the reading to sea level.

    To approximate the true barometric pressure reading from a corrected barometric pressure reading, use the following equation:

    True barometric pressure [mmHg] = Corrected barometric pressure [mmHg] – (0.025 * local altitude [feet] )

    To use the YSI 4010 MultiLab IDS meter with PC-BOD, the appropriate driver, computer settings, and software settings must be applied.  Download the Technical Bulletin for more details.

    There are several common methods to test wastewater and drinking water for organic pollutants, natural and chemical.  Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD) and, Total Organic Carbon (TOC) compromise the three main methods of testing water samples.  BOD and COD methods differ from TOC because they measure the amount of oxygen that is depleted by organic species in water. Moreover, TOC is a measure of all carbon (both organic and inorganic), rather than the oxygen that is reduced by these species.  As written by a TOC manufacturer, “TOC on its own sheds no light on the oxidizability of the measured carbon or the amount of oxygen needed for its biodegradation.  Specific to COD, it measures the reactive fraction of the TOC.  This is also known as oxidizability in the European Union.

    View the full article: Comparison of COD, BOD and TOC Methods for organics, which summarizes the advantages and disadvantages of the COD, BOD and TOC methods, and compares them to the PeCOD method.

    • If all samples fail the final DO rule, the software will report the value for the sample with the highest dilution (lowest sample volume) and display a greater than (>) symbol.
    • If all samples in a set fail the depletion rule and the BOD of the bottle with the lowest dilution (highest sample volume) is greater than the minimum detection, the software will report the value for the sample with the lowest dilution (highest sample volume) and display a less than (<) symbol.
    • A less than symbol (<) will appear when all samples in a set have a depletion of less than that specified in the BOD Method.  If the BOD of the bottle with the lowest dilution (highest sample volume) is less than the minimum detection for a given sample condition, the average will be displayed as follows:
      • Diluted samples with no seed added < 2 mg/L
      • Diluted samples with seed added < 1 mg/L
      • Undiluted samples with no seed added < 0.1 mg/L
      • Undiluted sampled with seed added < 0.0 mg/L

    MANTECH systems utilize one of two dissolved oxygen probes from YSI for automated dissolved oxygen determination. The measuring ranges of both probes are listed below, along with a link to the complete specification sheets.

    YSI FDO 4410 IDS Sensor

    • Dissolved Oxygen Range: 0 to 20 mg/L
    • Air Saturation Range: 0 to 200%
    • Temperature Measurement Range: 0 to 50°C
    • Technical Specifications

    YSI ProOBOD Sensor

    • Dissolved Oxygen Range: 0 to 50 mg/L
    • Air Saturation Range: 0 to 500%
    • Temperature Measurement Range: Ambient 10 to 40°C; Compensation -5 to 50°C
    • Technical Specifications

    Visit the BOD system resource database